Enzyme-free signal amplification in the DNAzyme sensor via target-catalyzed hairpin assembly.
نویسندگان
چکیده
A simple, highly sensitive and enzyme-free DNAzyme sensor based on target-catalyzed hairpin assembly is developed, which permits detection of 0.1 pM target DNA. Furthermore, this DNAzyme sensor is capable of detecting target DNA in real samples because of its high selectivity.
منابع مشابه
A smart ZnO@polydopamine-nucleic acid nanosystem for ultrasensitive live cell mRNA imaging by the target-triggered intracellular self-assembly of active DNAzyme nanostructures† †Electronic supplementary information (ESI) available: Oligonucleotide sequences, DLS and zeta potential measurements, TEM images, absorption and fluorescence spectra, cytotoxicity assay and CLSM images. See DOI: 10.1039/c6sc04633a Click here for additional data file.
Efficient strategies for the ultrasensitive imaging of gene expression in living cells are essential in chemistry and cell biology. Here, we report a novel and efficient enzyme-free dual signal amplification strategy for live cell mRNA imaging by using a smart nucleic acid hairpin-based nanosystem. This nanosystem consists of a ZnO nanoparticle core, an interlayer of polydopamine and an outer l...
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عنوان ژورنال:
- Chemical communications
دوره 48 25 شماره
صفحات -
تاریخ انتشار 2012